2024-02-26 バース大学
＜関連情報＞
- https://www.bath.ac.uk/announcements/scientists-develop-biocompatible-fluorescent-spray-that-detects-fingerprints-in-ten-seconds/
- https://pubs.acs.org/doi/10.1021/jacs.3c11277
緑色蛍光タンパク質発色団を利用したポータブルシステムによる潜在指紋取得プローブ De Novo Green Fluorescent Protein Chromophore-Based Probes for Capturing Latent Fingerprints Using a Portable System
Nanan Ruan, Qianfang Qiu, Xiaoqin Wei, Jiajia Liu, Luling Wu, Nengqin Jia, Chusen Huang, and Tony D. James
Journal of the American Chemical Society Published:January 8, 2024
DOI:https://doi.org/10.1021/jacs.3c11277
Abstract
Rapid visualization of latent fingerprints, preferably at their point of origin, is essential for effective crime scene evaluation. Here, we present a new class of green fluorescent protein chromophore-based fluorescent dyes (LFP-Yellow and LFP-Red) that can be used for real-time visualization of LFPs within 10 s. Compared with traditional chemical reagents for LFPs, these fluorescent dyes are completely water-soluble, exhibit low cytotoxicity, and are harmless to users. Level 1–3 details of the LFPs could be clearly revealed through “off–on” fluorescence signal readout. Additionally, the fluorescent dyes were constructed based on an imidazolinone core and so do not contain pyridine groups or metal ions, which ensures that the DNA is not contaminated during extraction and identification after the LFPs are treated with the dyes. Combined with our as-developed portable system for capturing LFPs, LFP-Yellow and LFP-Red enabled the rapid capture of LFPs. Therefore, these green fluorescent protein chromophore-based probes provide an approach for the rapid identification of individuals who were present at a crime scene.
