2026-04-10 中国科学院（CAS）
＜関連情報＞
- https://english.cas.cn/newsroom/research-news/202604/t20260413_1156021.shtml
- https://www.sciencedirect.com/science/article/abs/pii/S1385894726034571
- https://www.sciencedirect.com/science/article/abs/pii/S0304389425033242
- https://www.sciencedirect.com/science/article/abs/pii/S2213343725034724
二段階バイオコンバージョンにより、有毒な侵略性植物であるアゲラティナ・アデノフォラを栄養豊富な肥料に変換：堆肥化とアメリカミズアブの幼虫による相乗的な解毒 Dual-phase bioconversion transforms toxic invasive Ageratina adenophora into nutrient-rich fertilizer: Synergistic detoxification by composting and black soldier fly larvae
Yousif Abdelrahman Yousif Abdellah, Xiaofei Shi, Dou Tingting, Jianou Gao, Shimei Yang, Fengming Zhang, Zhenyan Yang, Chengmo Yang, Elsiddig A.E. Elsheikh, Zhuyue Yan, Rizwan Khan, Rashid Mohamed Ahmed, Dong Liu, Fuqiang Yu
Chemical Engineering Journal Available online: 7 April 2026
DOI:https://doi.org/10.1016/j.cej.2026.175996
Graphical abstract
Highlights
- Sequential composting and bioconversion detoxify A. adenophora-related phytochemicals.
- Black soldier fly larvae (BSFL) reduce key phytochemicals DTD and HHO by over 63%.
- Bioavailable heavy metals Cd and Pb were decreased by 67% and 54% in the biofertilizer.
- Final fertilizer showed 30% higher total nitrogen and enriched humic acid by 24%.
- Antibiotic-resistance genes and pathogenicity/virulence factors decreased by 1.6 to 2 times.
Abstract
Ageratina adenophora (AA), a neotropical invasive plant, produces toxic sesquiterpenes that disrupt ecosystems and impede conventional recycling. Here, a two-stage bioconversion platform was demonstrated: 56 days of co-composting (up to 10% dry weight AA), followed by 28 days of black soldier fly larvae (BSFL) bioconversion, which transforms this hazardous biomass into a safe, nutrient-rich agricultural amendment. Composting reduced key phytochemicals[4, 7-dimethyl-1-(propan-2-ylidene)-1,4,4a,8a-tetrahydronaphthalene-2, 6-dione (DTD) and 6-hydroxy-5-isopropyl-3, 8-dimethyl-4a,5,6,7,8,8a-hexahydronaphthalen-2(1H)-one (HHO)] from ∼2000 to 220.8 mg/kg and ∼ 700 to 96.4 mg/kg, respectively. Subsequent BSFL treatment achieved an additional 65.2% and 63.5% reduction in these compounds, while larval weight gain increased by 70%, indicating residual phytochemicals stimulated, rather than inhibited, larval metabolism via direct activation of metabolic pathways. Metagenomic analysis revealed AA enrichment of Proteobacteria and Bacteroidota, accelerating lignocellulose breakdown and humification; final humic acid content was 25% higher than controls. The process decreased DTPA-extractable heavy metals (Cu, Cr, Zn, Cd, Pb) by >40%, meeting China’s Grade-II fertilizer safety limits. Antibiotic-resistance genes and virulence factors declined 1.6–2-fold, while total nitrogen and potassium increased by 30% and 8%, respectively. Principal component analysis and Mantel tests linked detoxification, metal stabilization, and nutrient enhancement to temperature, moisture, and shifts in the microbial community. This scalable, circular-economy system achieves a 90% reduction in biomass, generates market-ready fertilizer, and provides a sustainable strategy for managing invasive plants, mitigating pollutant risks, and advancing sustainable agriculture.
植物化学物質による解毒：堆肥化システムにおけるアゲラチナ・アデノフォラ施用が重金属の生物学的利用能、病原体、および抗生物質耐性遺伝子の除去に及ぼす影響 Phytochemical-driven detoxification: Impact of Ageratina adenophora application on heavy metal bioavailability, pathogens, and antibiotic resistance genes removal in composting systems
Yousif Abdelrahman Yousif Abdellah, Dou Tingting, Jianou Gao, Shimei Yang, Zhenyan Yang, Chengmo Yang, Ayodeji Bello, Elsiddig A.E. Elsheikh, Dong Liu, Fuqiang Yu
Journal of Hazardous Materials Available online: 6 November 2025
DOI:https://doi.org/10.1016/j.jhazmat.2025.140404
Highlights
- Higher A. adenophora application ratios (7.5–10 %) significantly reduced metal bioavailability.
- AA application suppressed pathogens (>90 %) via phytochemical-mediated antimicrobial activity.
- ARGs reduction in AA treatments is linked to AA-driven shifts in microbial structure.
- AA application increased TN, TP, and TK by ≥ 1.25-fold, ≥ 11-fold, and ≥ 3.60-fold, respectively.
Abstract
The invasive Ageratina adenophora (AA) shows promise for sustainable waste management by producing nutrient-rich organic fertilizer. However, its ability to reduce environmentally hazardous components remains unstudied. In this study, the role of AA (2.5 %–10 % w/w) in composting systems, focusing on its phytochemical-driven detoxification capabilities, including the bioavailability of heavy metals, pathogen suppression, and the removal of antibiotic resistance genes (ARGs), was evaluated. Results showed that AA treatments at 7.5 % and 10 % significantly decreased bioavailable Zn (80.4 %-84.4 %), Cu (94.6 %-97.8 %), Cr (59 %-73.9 %), and Pb (58.8 %-80.2 %) via humification and microbial biosorption. Pathogens (Xanthomonas campestris, Staphylococcus aureus, Salmonella enterica) were suppressed by ≥ 90 %, due to phytochemical-mediated antimicrobial activity. Key ARGs (NmcR, oleC, adel) declined by ≥ 90 % in 5 %–10 % AA treatments, while MexS and mlaf dropped by ≥ 75 % in the 10 % AA treatment, probably because of phytochemical-driven microbial community restructuring. AA also significantly enhanced nutrient retention, with total nitrogen (TN) increasing by ≥ 1.25-fold (reaching 2.15-fold in 10 % AA), total phosphorus (TP) by ≥ 11-fold (reaching 15.6-fold in 10 % AA), and total potassium (TK) by ≥ 3.60-fold (reaching 7.50-fold in 10 % AA). Simultaneously, the germination index rose to ≥ 95.3 %. Mantel tests showed strong negative correlations between phytochemicals, pathogens, and metal bioavailability, indicating an additional layer of suppression and microbial/chemical immobilization during composting. Further studies are needed to evaluate long-term ecological risks and compare AA with traditional amendments like biochar. Overall, the findings suggest AA’s potential for sustainable waste management and soil remediation.
使用済みキノコ培地を用いたアゲラティナ・アデノフォラの堆肥化：植物化学物質の分解、温室効果ガスの削減、および栄養循環の促進 Composting Ageratina adenophora with spent mushroom substrate: Phytochemical degradation, greenhouse gas mitigation, and enhanced nutrient cycling
Yousif Abdelrahman Yousif Abdellah, Dong Liu, Dou Tingting, Jianou Gao, Shimei Yang, Zhenyan Yang, Chengmo Yang, Elsiddig A.E. Elsheikh, Shanshan Sun, Ayodeji Bello, Fuqiang Yu
Journal of Environmental Chemical Engineering Available online: 18 August 2025
DOI:https://doi.org/10.1016/j.jece.2025.118776
Highlights
- The phytochemical levels significantly decreased over time in all composting groups.
- Phytochemicals shape the GHGs relative activities of microbes during composting.
- AA 7.5 % and AA 10 % groups displayed higher humic acid amounts (29.9–35.9 g/kg).
- Bacillus alleviates the phytotoxicity of A. adenophora by degrading its phytochemicals.
- Nutrient cycling dynamics exhibited enriched N, P, and S retention in AA groups.
Abstract
Ageratina adenophora (AA), an invasive plant rich in nutrients; however, it is hindered by phytochemicals and cannot be directly recycled for environmental sustainability. Composting AA offers a viable solution, yet its impact on greenhouse gas (GHG) emissions, nutrient cycling, and microbial dynamics remains uninvestigated. Here, composting AA at varying ratios (0–10 %) was explored, assessing phytochemical degradation, GHG emissions, nutrient transformation, microbial succession, and metabolic pathways. Composting significantly degraded phytochemicals (up to 90 %), with efficiency scaling as AA 10 % > 7.5 % > 5 % > 2.5 %. Remarkably, AA-treatments suppressed CH₄ and CO emissions by days 28–35, while significantly reducing N₂O via phytochemical-mediated inhibition of denitrifiers. CO₂ emissions rose dose-dependently, peaking during thermophilic phases. AA application enhanced humification, with 7.5–10 % groups yielding 29.9–35.9 g/kg humic acid (vs. 16.1 g/kg in control), confirming accelerated maturity. Bacillus abundance surged (up to 32.4 % in AA 10 %), implicating its role in phytochemical degradation through co-metabolic ring cleavage and aromatic transformation. Nutrient cycling (N, P, S) improved significantly, supported by upregulated metabolic pathways (from 48.4 to 54.9 %). Multivariate analysis revealed strong negative correlations between residual phytochemicals and GHGs, underscoring their regulatory role. This research offers the first evidence that controlled AA composting simultaneously mitigates GHG emissions, boosts nutrient retention, and detoxifies phytochemicals via microbial-driven processes. These outcomes advance sustainable invasive plant management by optimizing composting strategies to enhance the ecological benefit and support carbon neutrality.
