2026-10-14 理化学研究所
2種類の微粒子マーカーを導入したハムスター卵巣細胞内部のX線ライトシート顕微鏡画像
＜関連情報＞
- https://www.riken.jp/press/2026/20261014_1/index.html
- https://opg.optica.org/oe/fulltext.cfm?uri=oe-34-21-39559
非点滅型シンチレーションナノ粒子を用いたX線ライトシート顕微鏡による3次元局在化 3-dimensional localization using X-ray lightsheet microscopy with non-blinking scintillating nanoparticles
Sierra Dean, Hidekazu Takano, Shun-Min Yang, Hsiang-Hsin Chen, Xike Tian, Yeukuang Hwu, Hideo Yokota, Giorgio Margaritondo, Tetsuya Ishikawa, and Yoshiki Kohmura
Optics Express Published: October 12, 2026
DOI:https://doi.org/10.1364/OE.601468
Abstract
We present two key achievements along the path towards widespread applications of nanometer-accuracy X-ray lightsheet microscopy. First, the thin volume of the X-ray lightsheet excitation eliminated the need for blinking scintillating nanoparticles (SciNPs) or fluorophores – typically required for super-resolution localization. This notably increased the reliability in localization analysis on the distribution of densely populated SciNPs on the surface of a silica microsphere. Second, we specifically achieved nanometer-level position accuracy in both axial (2 nm) and lateral (7 nm) coordinates using a three-dimensional localization method.
