2026-06-25 テキサスA&M大学
Researchers at Texas A&M University’s Institute for Quantum Science and Engineering have invented a laser technique that can directly measure the quantum forces shaping proteins, and how pharmaceutical drugs interact with them. Credit: Getty Images
＜関連情報＞
- https://stories.tamu.edu/news/2026/06/25/scientists-measure-hidden-quantum-forces-that-could-power-a-new-generation-of-pharmaceutical-drugs/
- https://www.science.org/doi/10.1126/sciadv.aeb3917
振動から機能へ：薬剤応答性タンパク質複合体におけるπ-πスタッキングの分光学的検出と定量 From vibrations to function: Spectroscopic detection and quantification of π-π stacking in drug-responsive protein complexes
Narangerel Altangerel, Esther J. Ocola, Benjamin W. Neuman, Vladislav V. Yakovlev, […] , and Marlan O. Scully
Science Advances Published:8 Apr 2026
DOI:https://doi.org/10.1126/sciadv.aeb3917
Abstract
Aromatic π-π stacking interactions are fundamental to protein architecture, molecular recognition, and drug efficacy, yet directly quantifying them under near-physiological conditions has remained challenging. Here, we use a recently developed spectroscopic platform, thermostable Raman interaction profiling (TRIP), that enables direct, label-free detection and quantification of aromatic π-π interactions in complex protein environments. Using the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) main protease (Mpro) as a biologically and clinically relevant model, we demonstrate that subtle changes in the phenylalanine benzene ring breathing (BRB) mode serve as a precise spectroscopic indicator of π-π stacking strength. This signal is highly responsive to both protein concentration-dependent dimerization and ligand-induced structural changes. Mpro forms a catalytically active dimer stabilized by a conserved aromatic triad (phenylalanine-140, histidine-163, and histidine-172), providing an ideal system to interrogate π-stacking at an important protein interface. Potent inhibitors MPI8 and nirmatrelvir produced the strongest BRB spectral shifts, broadening, and intensity changes, consistent with enhanced aromatic stacking and dimer stabilization, whereas halicin and VB-B-145 showed weaker engagement. BRB spectral changes also showed quantitative correlation with dimerization efficiency, published IC50 (median inhibitory concentration) values, and antiviral efficacy in A549-ACE2 cells. Complementary density functional theory revealed electron density rearrangements and vibrational coupling patterns unique to stacked aromatic residues. This integrated spectroscopic-computational approach enables quantitative probing of π-π stacking in native-like protein environments and positioning TRIP as a generalizable tool for designing drugs targeting aromatic protein-protein interfaces.
