2026-09-08 スイス連邦工科大学ローザンヌ校（EPFL）
Mitochondrial outer membrane imaged with SPIFFI (left) versus widefield microscopy (right). EPFL CC BY SA – 4.0
＜関連情報＞
- https://actu.epfl.ch/news/super-resolution-imaging-reveals-details-inside–2/
- https://www.nature.com/articles/s41592-026-03196-6
SPIFFIは、単一ショットでの超解像および多次元イメージングを可能にする SPIFFI enables single-shot super-resolution and multidimensional imaging
Wei Guo (郭威),Lely Feletti & Aleksandra Radenovic
Nature Methods Published:31 August 2026
DOI:https://doi.org/10.1038/s41592-026-03196-6
Abstract
Fluorescence super-resolution microscopy has advanced optical imaging into the nanoscale regime, transforming biological and interdisciplinary research. However, wide-field super-resolution techniques often compromise temporal resolution, thereby limiting the capture of rapid and transient biological events in living systems. Here we introduce spatial polarization-induced fluorescence fluctuation imaging (SPIFFI), a multichannel polarimetric method for single-shot super-resolution imaging and six-dimensional information extraction. By leveraging the inherently smaller point spread function under polarized detection and capturing polarization-dependent spatial fluctuations across multiplexed channels, SPIFFI achieves instant resolution enhancement from a single exposure. This capability substantially enhances the feasibility of volumetric live-cell super-resolution imaging. Moreover, SPIFFI images can integrate seamlessly with existing fluctuation-based methods for further postprocessing and resolution improvement. We demonstrate the versatility of SPIFFI through experiments on both fixed and live cells, capturing rapid subcellular dynamics and enabling high-throughput, multidimensional imaging beyond the diffraction limit. SPIFFI thus offers a practical and robust platform for real-time super-resolution imaging in biological research.
