木質胸筋症候群に関する知識を深める(Advancing knowledge on wooden breast syndrome)

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2024-05-07 デラウェア大学 (UD)

デラウェア州の大学の研究チームは、ブロイラーチキンに見られる「木質胸筋症候群」の解析を進めています。この症状は胸肉が硬くなるもので、アメリカの養鶏業者に年間少なくとも2億ドルの損失をもたらしています。研究により、脂肪を多く含んだ白血球が鶏の静脈周辺で炎症を引き起こし、胸肉の品質低下につながることが判明しました。また、この症状は遺伝的に大きな胸筋を持つように品種改良されたチキンに発生すると考えられています。この発見は、将来の繁殖戦略の改善や病気の早期診断に貢献する可能性があります。

<関連情報>

ブロイラー鶏の木質胸筋症候群の発症における血管周囲マクロファージ脂質代謝の変化を空間トランスクリプトミクスで明らかにした Spatial transcriptomics reveals alterations in perivascular macrophage lipid metabolism in the onset of Wooden Breast myopathy in broiler chickens

Ziqing Wang,Paul Khondowe,Erin Brannick & Behnam Abasht

Scientific Reports  Published:11 February 2024

DOI:https://doi.org/10.1038/s41598-024-53904-5

木質胸筋症候群に関する知識を深める(Advancing knowledge on wooden breast syndrome)

Abstract

This study aims to use spatial transcriptomics to characterize the cell-type-specific expression profile associated with the microscopic features observed in Wooden Breast myopathy. 1 cm3 muscle sample was dissected from the cranial part of the right pectoralis major muscle from three randomly sampled broiler chickens at 23 days post-hatch and processed with Visium Spatial Gene Expression kits (10X Genomics), followed by high-resolution imaging and sequencing on the Illumina Nextseq 2000 system. WB classification was based on histopathologic features identified. Sequence reads were aligned to the chicken reference genome (Galgal6) and mapped to histological images. Unsupervised K-means clustering and Seurat integrative analysis differentiated histologic features and their specific gene expression pattern, including lipid laden macrophages (LLM), unaffected myofibers, myositis and vasculature. In particular, LLM exhibited reprogramming of lipid metabolism with up-regulated lipid transporters and genes in peroxisome proliferator-activated receptors pathway, possibly through P. Moreover, overexpression of fatty acid binding protein 5 could enhance fatty acid uptake in adjacent veins. In myositis regions, increased expression of cathepsins may play a role in muscle homeostasis and repair by mediating lysosomal activity and apoptosis. A better knowledge of different cell-type interactions at early stages of WB is essential in developing a comprehensive understanding.

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