2025-01-23 バッファロー大学
＜関連情報＞
- https://www.buffalo.edu/news/releases/2025/01/bacteria-found-to-eat-forever-chemicals.html
- https://www.sciencedirect.com/science/article/abs/pii/S0048969724085061
Labrys portucalensis F11によるPFASの生分解：鎖短縮の証拠とPFOS、6:2 FTS、5:3 FTCAの代謝物の同定 PFAS biodegradation by Labrys portucalensis F11: Evidence of chain shortening and identification of metabolites of PFOS, 6:2 FTS, and 5:3 FTCA
Mindula K. Wijayahena, Irina S. Moreira, Paula M.L. Castro, Sarah Dowd, Melissa I. Marciesky, Carla Ng, Diana S. Aga
Science of The Total Environment Available online: 4 January 2025
DOI:https://doi.org/10.1016/j.scitotenv.2024.178348
Graphical abstract
Highlights
- Bacterial defluorination and chain shortening of PFOS, 6:2 FTS, 5:3 FTCA occurred
- PFOS removal of up to 96 % was observed after a 194-day incubation period
- After 100 day-incubation, up to 58 % of 5:3 FTCA and 21 % of 6:2 FTS were removed
- Several defluorinated PFOS metabolites were detected during biodegradation
Abstract
The biodegradation of three per- and polyfluoroalkyl substances (PFAS), namely perfluorooctane sulfonic acid (PFOS), 6:2-fluorotelomer sulfonic acid (6:2 FTS), and 5:3-fluorotelomer carboxylic acid (5:3 FTCA), were evaluated using Labrys portucalensis F11, an aerobic bacteria known to defluorinate fluorine-containing compounds. Cultures of L. portucalensis F11 were grown in minimal salts media and treated with 10,000 μg/L of individual PFAS as the sole carbon source in separate flasks. In PFOS-spiked media, several metabolites were detected, including perfluoroheptane sulfonic acid (PFHpS), perfluorohexane sulfonic acid (PFHxS), perfluorohexanoic acid (PFHxA), perfluoropentanoic acid (PFPeA), perfluorobutanoic acid (PFBA), and perfluoropropanoic acid (PFPrA). After 194-day incubation three de-fluorinated metabolites were identified: PFOS-F (m/z = 480.940, PFOS-2F (m/z = 462.980), and unsaturated PFOS-3F (m/z = 442.943). During the biodegradation of 5:3 FTCA, the following metabolites were observed: PFHxA, PFPeA, PFBA, PFPrA, and two fluorotelomer unsaturated carboxylic acids (5:3 FTUCA and 7:2 FTUCA). The biodegradation of 6:2 FTS was slower, with only 21 % decrease in concentration observed after 100 days, and subsequent formation of 4:2 FTS. On the contrary, 90 % of PFOS and 58 % of 5:3 FTCA were degraded after 100 days. These results indicate that L. portucalensis F11 can be potentially used for PFAS biodegradation in contaminated environments.
