2025-06-17 カリフォルニア工科大学（Caltech）
＜関連情報＞
- https://www.caltech.edu/about/news/speed-snap-science-solving-for-molecular-details-in-a-flash
- https://www.nature.com/articles/s41467-025-60072-1
超高速偏光異方性イメージングによる蛍光分子のシングルショット2次元ナノサイズマッピング Single-shot two-dimensional nano-size mapping of fluorescent molecules by ultrafast polarization anisotropy imaging
Peng Wang,Yogeshwar Nath Mishra,Florian J. Bauer,Murthy S. Gudipati & Lihong V. Wang
Nature Communications Published:30 May 2025
DOI:https://doi.org/10.1038/s41467-025-60072-1
Abstract
Molecular size plays a crucial role in determining the physical, chemical, and biological properties of substances. However, traditional fluorescence polarization anisotropy methods struggle to capture fast transient processes or provide plane-specific details. To overcome these limitations, we introduce Compressed Ultrafast Planar Polarization Anisotropy Imaging (CUP2AI). This approach combines femtosecond laser-sheet illumination, molecular rotational diffusivity, and compressed sensing to enable real-time, non-invasive, wide-field anisotropy measurements in both liquid and gas phases. CUP2AI captures 2D molecular size mapping in a single acquisition, granting unprecedented insights into dynamic events across various excitation modes (i.e. both one- and two-photon) and environmental conditions. It enables mapping of molecular volume (500 ų–80,000 ų) and hydrodynamic diameter (10 Å–50 Å) based on anisotropy lifetimes. We imaged fluorescein-conjugated dextran in water and polycyclic aromatic hydrocarbons in flames. CUP2AI holds transformative potential for applications ranging from molecular biology and drug design to nanoparticle formation.
