2025-05-07 オランダ・デルフト工科大学（TUDelft）
＜関連情報＞
- https://www.tudelft.nl/en/2025/tnw/mitigating-laughing-gas-emissions-from-wastewater
- https://www.nature.com/articles/s44221-025-00430-x
長期的なマルチメタオミクスにより、廃水処理中の季節的なN2O排出の生態生理学的制御が解明される Long-term multi-meta-omics resolves the ecophysiological controls of seasonal N2O emissions during wastewater treatment
Nina Roothans,Martin Pabst,Menno van Diemen,Claudia Herrera Mexicano,Marcel Zandvoort,Thomas Abeel,Mark C. M. van Loosdrecht & Michele Laureni
Nature Water Published:07 May 2025
DOI:https://doi.org/10.1038/s44221-025-00430-x
Abstract
Nitrous oxide (N2O) is the third most important greenhouse gas and originates primarily from natural and engineered microbiomes. Effective emission mitigations are currently hindered by the largely unresolved ecophysiological controls of coexisting N2O-converting metabolisms in complex communities. To address this, we used biological wastewater treatment as a model ecosystem and combined long-term metagenome-resolved metaproteomics with ex situ kinetic and full-scale operational characterization over nearly 2 years. By leveraging the evidence independently obtained at multiple ecophysiological levels, from individual genetic potential to actual metabolism and emergent community phenotype, the cascade of environmental and operational triggers driving seasonal N2O emissions has ultimately been resolved. We identified nitrifier denitrification as the dominant N2O-producing pathway and dissolved O2 as the prime operational parameter, paving the way to the design and fostering of robust emission control strategies. This work exemplifies the untapped potential of multi-meta-omics in the mechanistic understanding and ecological engineering of microbiomes towards reducing anthropogenic impacts and advancing sustainable biotechnological developments.
