2024-09-09 ブラウン大学
＜関連情報＞
- https://www.brown.edu/news/2024-09-09/mass-spectrometry
- https://www.nature.com/articles/s41467-024-51455-x
ナノポアイオン源は、アミノ酸やペプチドの個々のイオンを直接高真空中に供給する Nanopore ion sources deliver individual ions of amino acids and peptides directly into high vacuum
Nicholas Drachman,Mathilde Lepoitevin,Hannah Szapary,Benjamin Wiener,William Maulbetsch & Derek Stein
Nature Communications Published:04 September 2024
DOI:https://doi.org/10.1038/s41467-024-51455-x
Abstract
Electrospray ionization is widely used to generate vapor phase ions for analysis by mass spectrometry in proteomics research. However, only a small fraction of the analyte enters the mass spectrometer due to losses that are fundamentally linked to the use of a background gas to stimulate the generation of ions from electrosprayed droplets. Here we report a nanopore ion source that delivers ions directly into high vacuum from aqueous solutions. The ion source comprises a pulled quartz pipette with a sub-100 nm opening. Ions escape an electrified meniscus by ion evaporation and travel along collisionless trajectories to the ion detector. We measure mass spectra of 16 different amino acid ions, post-translationally modified variants of glutathione, and the peptide angiotensin II, showing that these analytes can be emitted as desolvated ions. The emitted current is composed of ions rather than charged droplets, and more than 90% of the current can be recovered in a distant collector.
