新しい質量分析コンボは、自然界の未知の化学物質の宇宙を利用できる可能性がある。 New mass spectrometry combo offers promise for tapping nature’s unknown chemical universe
2023-06-27 パシフィック・ノースウェスト国立研究所(PNNL)
◆彼らは質量分析法を使用して化学物質の特性を測定し、未知の化合物を同定する新しい方法を模索しています。最新の研究では、2つの高分解能機器を組み合わせたシステムを開発し、化学物質を詳細に分析することに成功しました。これにより、より迅速で正確な情報が得られるようになりました。この研究は、PNNLのm/qイニシアチブによって支援されました。
＜関連情報＞
- https://www.pnnl.gov/news-media/chemists-are-hunt-other-99-percent
- https://pubs.acs.org/doi/10.1021/acs.analchem.3c00881
ロスレスイオン操作のためのデュアルゲート構造-超高分解能分子分析のためのイオンモビリティーオービトラップ質量分析プラットフォーム A Dual-Gated Structures for Lossless Ion Manipulations-Ion Mobility Orbitrap Mass Spectrometry Platform for Combined Ultra-High-Resolution Molecular Analysis
Adam L. Hollerbach, Yehia M. Ibrahim, Vanessa Meras, Randolph V. Norheim, Adam P. Huntley, Gordon A. Anderson, Thomas O. Metz, Robert G. Ewing, and Richard D. Smith
Analytical Chemistry Published:June 12, 2023
DOI:https://doi.org/10.1021/acs.analchem.3c00881
Abstract
High-resolution ion mobility spectrometry-mass spectrometry (HR-IMS-MS) instruments have enormously advanced the ability to characterize complex biological mixtures. Unfortunately, HR-IMS and HR-MS measurements are typically performed independently due to mismatches in analysis time scales. Here, we overcome this limitation by using a dual-gated ion injection approach to couple an 11 m path length structures for lossless ion manipulations (SLIM) module to a Q-Exactive Plus Orbitrap MS platform. The dual-gate setup was implemented by placing one ion gate before the SLIM module and a second ion gate after the module. The dual-gated ion injection approach allowed the new SLIM-Orbitrap platform to simultaneously perform an 11 m SLIM separation, Orbitrap mass analysis using the highest selectable mass resolution setting (up to 140 k), and high-energy collision-induced dissociation (HCD) in ∼25 min over an m/z range of ∼1500 amu. The SLIM-Orbitrap platform was initially characterized using a mixture of standard phosphazene cations and demonstrated an average SLIM CCS resolving power (RpCCS) of ∼218 and an SLIM peak capacity of ∼156, while simultaneously obtaining high mass resolutions. SLIM-Orbitrap analysis with fragmentation was then performed on mixtures of standard peptides and two reverse peptides (SDGRG1+, GRGDS1+, and RpCCS = 305) to demonstrate the utility of combined HR-IMS-MS/MS measurements for peptide identification. Our new HR-IMS-MS/MS capability was further demonstrated by analyzing a complex lipid mixture and showcasing SLIM separations on isobaric lipids. This new SLIM-Orbitrap platform demonstrates a critical new capability for proteomics and lipidomics applications, and the high-resolution multimodal data obtained using this system establish the foundation for reference-free identification of unknown ion structures.
