2025-07-18 岩手生物工学研究センター,量子科学技術研究開発機構
得られた突然変異系統の例
左 突然変異系統 右 元品種
八重咲き 一重咲き
雄しべが花弁化し、八重咲きになり、花サイズが約1.5倍になった。
＜関連情報＞
- https://www.qst.go.jp/site/press/20250718.html
- https://www.jstage.jst.go.jp/article/plantbiotechnology/advpub/0/advpub_25.0501a/_article
イオンビーム照射葉からの再生による突然変異誘発と八重咲きリンドウの作出 Mutagenesis and production of double-flowered gentians via regeneration from ion beam-irradiated leaves
Masahiro Nishihara , Akiko Hirabuchi, Akira Abe, Motoki Shimizu, Fumina Goto, Chiharu Yoshida, Takashi Shimokawa, Suguru Ozawa, Zenbi Naito, Keiichiro Nemoto
Plant Biotechnology Published: July 17, 2025
DOI:https://doi.org/10.5511/plantbiotechnology.25.0501a
Abstract
Gentians are important ornamental plants, and gentian cultivars have been actively bred for decades. However, limited genetic resources are currently available for breeding; therefore, artificial mutagenesis has been applied to generate mutants. In this study, we developed a simple and efficient regeneration-mediated method for ion beam mutagenesis in the Japanese gentian hybrid cultivar ｀Albireo’ (Gentiana scabra × G. triflora). Carbon and neon ion species were tested. Effect of ion beam irradiation on callus formation from leaves was initially evaluated. Tissue culture was then continued, adventitious shoots were induced from calli, and many regenerated plants were obtained. These plants were cultivated until flowering, and two cultivated lines exhibiting a double-flowered phenotype were identified from leaves exposed to 9 and 12 Gy of neon ion beam irradiation among approximately 200 individuals. We analyzed one line derived from irradiation with 9 Gy of neon ions, named Ne9Gy#34, in detail. The agamous gene (AG1), previously identified as the gene responsible for the double-flower phenotype in gentians, was not amplified in the G. scabra allele by genomic polymerase chain reaction. Moreover, next-generation sequencing also indicated that the reads were mapped to the genomic region of the G. triflora AG1 but not to that of G. scabra, suggesting that the deletion of G. scabra AG1 led to the double-flowered phenotype. Ne9Gy#34 also exhibited increased flower size, suggesting additional mutations in genes other than AG1. In summary, the developed regeneration-mediated method represents a promising approach for inducing gentian mutagenesis and efficiently producing novel traits in this plant.
