2025-08-05 オークリッジ国立研究所(ORNL)
Scientists developed a new approach to study the molecular makeup of the plant soil environment, yielding data that can drive innovations for better energy and food crops. Image: Philip Gray/ORNL, U.S. Dept. of Energy
<関連情報>
- https://www.ornl.gov/news/study-unearths-rich-data-better-bioenergy-crops
- https://onlinelibrary.wiley.com/doi/10.1111/pce.70004
- https://www.nature.com/articles/s41597-024-03069-7
木本性多年草ポプラ(Populus trichocarpa)における動的根圏微生物沈着 Dynamic Rhizodeposition in the Woody Perennial Populus trichocarpa
Manasa R. Appidi, Sameer Mudbhari, Kevin Cope, Sara S. Jawdy, Dana L. Carper, Edanur Öksüz, Xianghu Wang, Timothy Tschaplinski, Mingxun Wang, Robert L. Hettich, Udaya Kalluri, Paul E. Abraham
Plant, Cell & Environment Published: 22 June 2025
DOI:https://doi.org/10.1111/pce.70004
ABSTRACT
Plants undergo physiological and metabolic changes that release specific molecules into the surrounding soil, a process collectively known as rhizodeposition. These compounds play crucial roles in plant-microbe-soil interactions, such as supporting plant development and resilience in changing environments. Under nutrient-limited conditions, these plant-derived compounds modify the rhizosphere environment, mobilizing otherwise inaccessible nutrients and recruiting stress-adaptive microbial communities that support stress resilience. Currently, the chemical diversity of rhizodeposition has yet to be fully realized but is expected to be a complex mixture that includes soluble organic compounds excreted from root cells, along with products of root cell turnover, sloughed-off root cap and border cells, and mucilage. Here, we developed a methodological and conceptual framework for an in-depth measurement of rhizodeposition through critical advancements in untargeted metabolomics. This approach provided foundational insights into the dynamic changes in rhizodeposition for the woody perennial Populus trichocarpa and rhizodeposit profiles varying by genotype, time, location, and environment. More broadly, this study provides a framework that will help formulate the next steps to effectively study rhizodeposition.
ポプラの遺伝子型と環境を横断したメタゲノム、メタボローム、トランスクリプトームの統合的調査 An integrated metagenomic, metabolomic and transcriptomic survey of Populus across genotypes and environments
Christopher Schadt,Stanton Martin,Alyssa Carrell,Allison Fortner,Dan Hopp,Dan Jacobson,Dawn Klingeman,Brandon Kristy,Jana Phillips,Bryan Piatkowski,Mark A. Miller,Montana Smith,Sujay Patil,Mark Flynn,Shane Canon,Alicia Clum,Christopher J. Mungall,Christa Pennacchio,Benjamin Bowen,Katherine Louie,Trent Northen,Emiley A. Eloe-Fadrosh,Melanie A. Mayes,Wellington Muchero,… Mitchel Doktycz
Scientific Reports Published:05 April 2024
DOI:https://doi.org/10.1038/s41597-024-03069-7
Abstract
Bridging molecular information to ecosystem-level processes would provide the capacity to understand system vulnerability and, potentially, a means for assessing ecosystem health. Here, we present an integrated dataset containing environmental and metagenomic information from plant-associated microbial communities, plant transcriptomics, plant and soil metabolomics, and soil chemistry and activity characterization measurements derived from the model tree species Populus trichocarpa. Soil, rhizosphere, root endosphere, and leaf samples were collected from 27 different P. trichocarpa genotypes grown in two different environments leading to an integrated dataset of 318 metagenomes, 98 plant transcriptomes, and 314 metabolomic profiles that are supported by diverse soil measurements. This expansive dataset will provide insights into causal linkages that relate genomic features and molecular level events to system-level properties and their environmental influences.
