2024-11-06 マサチューセッツ大学アマースト校
＜関連情報＞
- https://www.umass.edu/news/article/new-pfas-testing-method-created-umass-amherst
- https://www.science.org/doi/10.1126/sciadv.adp8134
シクロデキストリンを介した生体ナノ細孔内でのホスト-ゲスト相互作用によるペルフルオロアルキルおよびポリフルオロアルキル物質の1分子プロファイリング Single-molecule profiling of per- and polyfluoroalkyl substances by cyclodextrin mediated host-guest interactions within a biological nanopore
Xiaojun Wei, Aditya Choudhary, Leon Y. Wang, Lixing Yang, […], and Chang Liu
Science Advances Published:6 Nov 2024
DOI:https://doi.org/10.1126/sciadv.adp8134
Abstract
Biological nanopores are increasingly used in molecular sensing due to their single-molecule sensitivity. The detection of per- and polyfluoroalkyl substances (PFAS) like perfluorooctanoic acid and perfluorooctane sulfonic acid is critical due to their environmental prevalence and toxicity. Here, we investigate selective interactions between PFAS and four cyclodextrin (CD) variants (α-, β-, γ-, and 2-hydroxypropyl-γ-CD) within an α-hemolysin nanopore. We demonstrate that PFAS molecules can be electrochemically sensed by interacting with a γ-CD in a nanopore. Using HP-γ-CDs with increased steric resistance, we can identify homologs of the perfluoroalkyl carboxylic acid and the perfluoroalkyl sulfonic acid families and detect common PFAS in drinking water at 0.4 to 2 parts per million levels, which are further lowered to 400 parts per trillion by sample preconcentration. Molecular dynamics simulations reveal the underlying chemical mechanism of PFAS-CD interactions. These insights pave the way toward nanopore-based in situ detection with promises in environmental protection against PFAS pollution.
